customized bio-plex pro™ human cytokine arrays  (Bio-Rad)

Journal: Pregnancy hypertension

Article Title: Tumor Necrosis Factor-alpha Blockade Improves Uterine Artery Resistance, Maternal Blood Pressure, and Fetal Growth in Placental Ischemic Rats.

doi: 10.1016/j.preghy.2021.05.002

Figure Lengend Snippet: Fig. 3. Effect of TNF-α Blockade on Circulating and Placental Angiogenic Factors. On Gestation Day (GD) 14, either the Reduced Uterine Perfusion Pressure (RUPP) procedure or a Sham procedure was performed on pregnant Sprague Dawley rats. On GD 15 and 18, vehicle or 0.4 mg/kg Etanercept was injected i.p. in a subset of Sham and RUPP rats. On GD 19, blood and placentas were collected under isoflurane anesthesia and processed for further analysis. Circulating sFlt-1(A) and VEGF(B) were measured via ELISA and the sFlt-1:VEGF ratio (C) is shown. Placental VEGF (D) was measured using the Bio-Plex Pro Rat Cytokine Immunoassay Kit. Sham: n = 9–10; RUPP: n = 10; Sham + ETA: n = 9–10; RUPP + ETA: n = 9–10. All data are expressed as mean ± SEM. Statistical analyses were performed using two-way ANOVA with multiple comparisons followed by Tukey’s post-hoc test. *p < 0.05 versus Sham; #p < 0.05 vs RUPP.

Article Snippet: Circulating and placental TNF-α, IFN-γ, IL-6, and placental VEGF were measured in collected serum using a custom Bio-Plex Pro Rat Cytokine Immunoassay Kit (Bio-Rad, Hercules, CA) according to the manufacturer’s instructions Circulating VEGF was measured in collected serum via ELISA (Boster Biological Technology, Pleasanton, CA; Catalog# EK054) according to the manufacturer’s instructions.

Techniques: Injection, Enzyme-linked Immunosorbent Assay